List of Provided Resources

 

This strain originated from a Nigerian strain maintained at the Grainger lab (University of Virginia, USA). Dr. Asashima (The University of Tokyo) obtained the sixth generation of the Nigerian A2 strain used for genome sequencing by JGI as a generous gift from Dr. R. Grainger in 2003. This strain was propagated to the eleventh inbred generation in his laboratory and provided to IAB in 2014. The present maximum generation is the 15th.

I. Nigerian A (NA) strain

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Dr. K. Yasuda (Nara Institute of Science and Technology, Japan) obtained X. tropicalis frogs from the Grainger lab in 1996. He carried out several generations of inbreeding and provided specimens to IAB in 1998 and 2003. This strain was previously labeled “Yasuda”, but we renamed it “Nigerian H” because its mitochondrial haplotype is identical to Nigerian A. The present maximum generation is the 10th.

II. Nigerian H (Nigerian Hiroshima; NH) strain

This strain is Stock B used in [Uehara et al. (2002) Develop. Growth and Differ. 44: 427–436]. This line belongs to the same lineage as the NH line mentioned above. A total of 218 frogs kept in the Advanced Industrial Science and Technology (AIST) were transferred to IAB on October 16, 2012, and the line has been reared here since then. The present maximum generation is the 7th.

This strain originally came from a stock maintained by Dr. E. Amaya at the University of California, Berkeley, and subsequently maintained for a short period by Dr. T. Hyaes. The Golden strain was developed through artificial selection for fast growth and rapid sexual maturity in the Harland lab. Dr. M. Asashima obtained this strain from Dr. R. Harland in 2005 and provided specimens to IAB in 2009 and 2010. The present maximum generation is the 7th.

III. Golden (G) strain

This strain was taken from a wild population in Adipodoume, Ivory Coast. Adult males and females were presented to Dr. K. Yoshizato (IAB at that time) in 1998 by Drs. D. Rungger and H.R. Kobel at Université de Genève. The present maximum generation is the 4th.

IV. Ivory Coast (IC) strain

The Asashima strain is “Stock A” used in [Uehara et al. (2002) Develop. Growth and Differ. 44: 427–436]. This line was collected in Africa by an animal dealer, imported from the United States to Japan, and kept at The University of Tokyo. The present maximum generation is the 3rd.

VI. Asashima strain

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V. Nigerian S (NS) strain

List of Bioresources

For the inbreeding ratio and genetic relationships of inbred strains at IAB, as well as stocks from other institutions, refer to “Igawa et al. (2015) PLoS ONE 10(7): e0133963. doi:1371/journal. pone.0133963”.


In addition to the above strains, we maintain several gynogenetic diploid and triploid strains.

Non-living resources

• Plasmid cDNA clones for whole-mount in situ hybridization of X. tropicalis embryos

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Genomic DNA derived from X. tropicalis liver

Total RNA and cDNA from early embryos of X. tropicalis